Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAVEQQIVDYVASDFETGAE-QADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQVVTSAEQ-KNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF---CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDITRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKESRDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGILQISFKNQQDLLQAKRIIMSNTSYHCWIK 3GGO Chain:B ((34-312)) --QNVLIVGVGFMGGSFAKSLRRSGFKGKIYGYDINPESISKAVDLGIIDEGTTSIAKVEDFSPDFVMLSSPVRTFREIAKKLSYI-LSEDATVTDQGSVKGKLVYDLENILGKRFVGGHPIAGTEKSGVEYSLDNLYEGKKVILTPTKKTDKKRLKLVKRVWEDVGGVVEY--MSPELHDYVFGVVSHLPHAVAFALVDTLIHMSTPEVDLFKYPGGGFKDFTRIAKSDPIMWRDIFLENKENVMKAIEGFEKSLNHLKELIVREAEEELVEYLKEVKIKRME------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3GGO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1343 -27606 -20.56 -100.75 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain B : 0.71 3D Compatibility (PKB) : -20.56 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.454

(partial model without unconserved sides chains): PDB file : Tito_3GGO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3GGO-query.scw PDB file : Tito_Scwrl_3GGO.pdb: