Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKPEYLIHAEHLTVILCPLEKQH-KI---LEHLKK--------------EVL-VAKLDNFAIIQKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPS----------LLNCAAAELTSFEKVKLQLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSPYIDRLEPAG 3B60 Chain:D ((361-549)) -NINLKIPAGKTVALVGRSGSGKSTIASLITRFYDIDEGHILMDGHDLREYTLASLRNQVALVSQNVHLFND-TVANNIAYARTEEYSREQIEEAARMAYAMDFINKMDNGLDTIIGENGVLLSGGQRQRIAIARALLRDSPILILDEATSALDTESERAIQAALDELQK--NRTSLVIAHRLSTIE--QADEIV---

General information: TITO was launched using: RESULT: Template: 3B60.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 658 -5718 -8.69 -35.74 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain D : 0.66 3D Compatibility (PKB) : -8.69 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.399

(partial model without unconserved sides chains): PDB file : Tito_3B60.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3B60-query.scw PDB file : Tito_Scwrl_3B60.pdb: