Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEINYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLGQVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVTIPVDLGFEEID-EQPFSTAHTHKTSVIL-PEEKDLLAALPYLEKAKKPVLYVGQGTRNGFPQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGSDFPFG---RAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSWYLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIRLLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQVKYQLPIINVVFSNDSFGFIEAEQEDTEQKKF-GVFLEDADFGKVGEALGANSFTVTEYSQLRFAFDAAA--KSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLPVLNELYEKRL 2EZT Chain:B ((9-583)) -TNILAGAAVIKVLEAWGVDHLYGIPGGSINSIMDALSAERDRIHYIQVRHEEVGAMAAAADAKLTGKIGVCFGSAGPGGTHLMNGLYDAREDHVPVLALIGQFGTTGMNMDTFQEMNENPIYADVADYNVTAVNAATLPHVIDEAIRRAYAHQGVAVVQIPVDLPWQQIPAEDWYASANSYQTPLLPEPDVQAVTRLTQTLLAAERPLIYYGIGARKAGKELEQLSKTLKIPLMSTYPAKGIVADRYPAYLGSANRVAQKPANEALAQADVVLFVGNNYPFAEVSKAFKN-TRYFLQIDIDPAKLGKRHKTDIAVLADAQKTLAAILAQVSERESTPWWQANLANVKNWRAYLASLEDKQEGPLQAYQVLRAVNKIAEPDAIYSIDVGDINLNANRHLKLTPSNRHITSNLFATMGVGIPGAIAAKLNYPERQVFNLAGDGGASMTMQDLATQVQYHLPVINVVFTNCQYGWIKDEQEDTNQNDFIGVEFNDIDFSKIADGVHMQAFRVNKIEQLPDVFEQAKAIAQHEPVLIDAVITGDRPLPAEKLRLDSAMSSAADIEAFKQRYEAQDLQPLS-------

General information: TITO was launched using: RESULT: Template: 2EZT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 3550 -30178 -8.50 -53.22 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain B : 0.87 3D Compatibility (PKB) : -8.50 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.87 QMean score : 0.526

(partial model without unconserved sides chains): PDB file : Tito_2EZT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2EZT-query.scw PDB file : Tito_Scwrl_2EZT.pdb: