Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGS-YSGIMGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLVFPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWLDQVIFSNNPLELVNELRAVSIKNQLLLKEAK 1T35 Chain:E ((2-184)) KTICVFAGSNPGGNEAYKRKAAELGVYMAEQGIGLVYGGSRV-GLMGTIADAIMENGGTAIGVMPSGLFSGEVVHQNLTELIEVNGMHERKAKMSELADGFISMPGGFGTYEELFEVLCWAQIGI-HQKPIGLYNVNGYFEPMMKMVKYSIQEGFSNESHLKLIHSSSRPDELIEQMQNYSYPIL-------

General information: TITO was launched using: RESULT: Template: 1T35.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain E - contact count / total energy / energy per contact / energy per residue : 856 -50827 -59.38 -279.27 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain E : 0.81 3D Compatibility (PKB) : -59.38 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.526

(partial model without unconserved sides chains): PDB file : Tito_1T35.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1T35-query.scw PDB file : Tito_Scwrl_1T35.pdb: