Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNKEMSQTIKVQKMIDDLTHGIDSQADKIIKELQGQKVKDAKMLLKTINFE-MNPTKRKLADVLEEKLASA-INEQELLFETDTFNS---------------------------------------------------------------------------------------- 5MMI Chain:T ((24-195)) --PEPGKCDEITTRGYSISMSVD-KARRVIDQIRGRSYAETLMILELMPYRACYPIFKLIYSAAANASHNKQFNKANLIISKAEVNKGITLKKVKPRARGRSYMIKRPTCHITIVLRDITHFDSYDKFLESLTPKKLIALLGLMSTGRRRELLCGRFRENHKIKSFLYKIALFKR

General information: TITO was launched using: RESULT: Template: 5MMI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain T - contact count / total energy / energy per contact / energy per residue : 266 -13241 -49.78 -161.47 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain T : 0.61 3D Compatibility (PKB) : -49.78 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.476

(partial model without unconserved sides chains): PDB file : Tito_5MMI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5MMI-query.scw PDB file : Tito_Scwrl_5MMI.pdb: