Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -FGRRRDGRMPPWEWVTWKD------FCRLTLKKGDEIQSQSKWVAKPGPIGILIQPGVYESFKTDKDCNPKIRRGKLPDG--YTLHGQLKYITPDLASMYNRVLDDTKWWPFDSQEAPQTSTTVEKSL 5J4F Chain:A ((22-119)) EVVHFLEGVCFEKLHIEVLNENSSHKEIRICMPKGAVMDKHKA----PGAISVQVLEGKIVFEVGD-------EKIEMPKGALISLEAQVLHRLDALENSVIRLSLSKK--------------------

General information: TITO was launched using: RESULT: Template: 5J4F.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 351 21635 61.64 243.09 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : 61.64 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.159

(partial model without unconserved sides chains): PDB file : Tito_5J4F.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5J4F-query.scw PDB file : Tito_Scwrl_5J4F.pdb: