Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQATTSHWITNRQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKVMFEIAGVSEEIAREALRLASHKLPVKCKFVKREAE 5O60 Chain:N ((1-136)) MLIPRKVKHRKQHHPEQRGIASGGTSVSFGDYGIQALEHAYITNRQIESARIAINRHIKRGGKVWINIFPDRPLTKKPAETRMGSGKGSPEWWVANVKPGRVLFELSYPDEKTARDALTRAIHKLPIKARIVTREE-

General information: TITO was launched using: RESULT: Template: 5O60.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain N - contact count / total energy / energy per contact / energy per residue : 602 -56926 -94.56 -418.57 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain N : 0.88 3D Compatibility (PKB) : -94.56 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.88 QMean score : 0.511

(partial model without unconserved sides chains): PDB file : Tito_5O60.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5O60-query.scw PDB file : Tito_Scwrl_5O60.pdb: