Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKWLQIHITVDQEQVEFTETLLMSLGAVSVTLDDAEDQALLEPLPGETPLWNKVIVTGIYQQDEQDPIDVDTLEAFLKAQLPDVPMRHEELEDQVWERAWMDYYEPIQIGEKFWIVPEWLEPPEADATNIKLDPGLAFGTGNHASTFLCLQWLGKTDVKNKIVIDYGCGSGILGVAALLLGAKKVYATDIDPQAVLATKQNAEL----NGVLDRLYVGLPEEFDQEFKPQQADVLVA----------NILAGPLMA---LAPEFAKLLKSDGDFALAGVIEEQVVDVSGVYSEFFDILDVEKREENWCRISGKRKTTN 5IS8 Chain:C ((55-177)) ----------------------------------------------------------------------------------------------------------------------------------------------------------HTDFKDKIVLDVGCGSGILSFFAAQAGARKIYAVEASTMA-----QHAEVLVKSNNLTDRIVV-IPGKVEEVSLPEQVDIIISEPMGYMLFNERMLESYLHAKKYLKPS-GNMFPTIGDVHLAPFTDEQL----------------------------------

General information: TITO was launched using: RESULT: Template: 5IS8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 484 -27807 -57.45 -262.33 target 2D structure prediction score : 0.44 Monomeric hydrophicity matching model chain C : 0.61 3D Compatibility (PKB) : -57.45 2D Compatibility (Sec. Struct. Predict.) : 0.44 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.437

(partial model without unconserved sides chains): PDB file : Tito_5IS8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5IS8-query.scw PDB file : Tito_Scwrl_5IS8.pdb: