Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAKKIDGYIKLQVPAGKANPSPPIGPALGQRGVNIMAFCKEFNAATQKVEPGLPIPVVITVYNDKSFTFIMKTPPASILLKKAAGIQKGSSVPNKTKVGKLTRAQLEEIATTKEPDLTGADLDARVRTIAGSARSMGLEVEL 3CJQ Chain:E ((1-137)) --KKVVAVVKLQLPAGKATPAPPVGPALGQHGANIMEFVAAFNAATANMGDAI-VPVEITIYADRSFTFVTKTPPASYLIR-------------------ITWEQVLEIAKQKMP------LEAAARMIAGSARSMGVEV--

General information: TITO was launched using: RESULT: Template: 3CJQ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain E - contact count / total energy / energy per contact / energy per residue : 426 -74527 -174.94 -665.42 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain E : 0.81 3D Compatibility (PKB) : -174.94 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.612

(partial model without unconserved sides chains): PDB file : Tito_3CJQ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3CJQ-query.scw PDB file : Tito_Scwrl_3CJQ.pdb: