Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKVWIDKLQQLQWQKLDRLSVVVLAILILWLCWKLASFFWLVIAPPQLMQFDRVELGSQQPQIPNISTFSLFNEPSANAAQESVNLELQGVMVGYPNRFSSAVIKIDNTAERYRVGETIGSTSYQLAEVYWDHVVLIQGNGSTRELQFKGLPNGLYQPMTPDASQQSATPSQPTEPMNTAQQALGQAIQQMQGNREQYLRDMGVSGNSGEGYEVTERTPTALRNKLGLRPGDRIVSLNGQAVGQGQTDVQLLEQARRAGQVKIEIKRGDQVMIIQQNF 5B6L Chain:A ((264-331)) -----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DGILVMRVLPGTPAERAGIRRGDVIVAVDGTPISDGARLQRIVEQAGLNKALKLDLLRGDRRLSLTVQ-

General information: TITO was launched using: RESULT: Template: 5B6L.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 230 -25396 -110.42 -373.47 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.55 3D Compatibility (PKB) : -110.42 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.55 QMean score : 0.482

(partial model without unconserved sides chains): PDB file : Tito_5B6L.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5B6L-query.scw PDB file : Tito_Scwrl_5B6L.pdb: