Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSIVERPSYTSKDVEVTSREPLFSGFIQVEKVSLRHRLFNQSEYTPVLQRELVHRPEAAGVLLYNDQKQQFALIEQFRVGALDDSHSPWQL-EIIAGVLDGNESPESCIRCESLEESGCEVQDLEHLFSFYPSAGACSELFHLYVAETN----LPAVGGVFGVDNEGENIQLHLFSYSEIQTLLNSGRLRNAPVIMALQWLAQHSKTIINPKR 3O61 Chain:C ((38-184)) ------------------------------------------------KREVYDRGNGATILLYNTKKKTVVLIRQFRVATWVNGNESGQLIESCAGLLD-NDEPEVCIRKAAIEETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRA-------------EDIEVLELPFSQALEMIKTGEIRDGKTVLLLNYL------------

General information: TITO was launched using: RESULT: Template: 3O61.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 590 -26800 -45.42 -200.00 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain C : 0.75 3D Compatibility (PKB) : -45.42 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.416

(partial model without unconserved sides chains): PDB file : Tito_3O61.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3O61-query.scw PDB file : Tito_Scwrl_3O61.pdb: