Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKSLRKDKLANEFVKKINNFSKKIIFLPGADSQLQLDVLARQIVDSINRVDYFNILESRNICASRADPYSDLFDPIRAILFLKNQDYDEACWLAFLLVFTGENYHSKWKFTKLLYGNLGKSPMMTWKNVNNNITLISSWVDNYKQSDYKIKFGNHRKYVSLKQLKEAVSTYIQWINNNGGHNNLFRSTHISNKELFNQLYKTLPIYTFGRLGKFDYLSMLYKTGLANIKADNCYIAGSTGPRKGAKLLFGNKSDKELDKIAIQLADFLGIGYQEMEDALCNWQKSPDKYEYYIG 1TFJ Chain:A ((16-219)) EHLLRPLP---------------------ADKQIETGPFLEAVS---HLPPFFDCLGS----PVFTPIKADISGNITKIKAVYDTNPTKFRTLQNILEVEKEMYGAEWP-------KVGATLALMW--LKRGLRFIQVFLQSICDGERDENHPNLIRVNATKAYEMALKKYHGWIVQKIFQAALYAAPY-----------------------KSDFLKALSKGQNVT------------------------------EEECLEKVRLFLVNYTATIDVIYEMYTRMNAELNYKV

General information: TITO was launched using: RESULT: Template: 1TFJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 839 -82147 -97.91 -402.68 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -97.91 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.267

(partial model without unconserved sides chains): PDB file : Tito_1TFJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1TFJ-query.scw PDB file : Tito_Scwrl_1TFJ.pdb: