Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKLLKRQILRRRRHFMIFNQDEIYVIAEIGVNHNGSVDLAKELILKAKDCGANAVKFQTFKADSLLSDQTEMAAYQKENTGSSQSQLELVKSLELTYEQTEEIKKFCDEQKITFISTPFDSDSLKFLVNEIDVPYLKVSSADISNLPFLYEIACSKKHVILSTGTASLGDIEQALSVFAFVIDKGTEVQPSQQLFREAYSKISVRKQLKQQVSILHCVTQYPALFEESNLKAISTLKNVF-DLATGFSDHTLDE-YAAVISVSLGARIFEKHITLDKTMAGPDHAASMEPNEFKHYVEILHKTHAALGDG-------------IKFMLEQESDNYYLVRRSIVAKTDIAEGELLTADNITTKRAGR-V-CLEPNKYWD-VIGTKAKRSFKKNDFIEI 1VLI Chain:A ((19-372)) ------------ANKTVGKDAPVFIIAEAGINHDGKLDQAFALIDAAAEAGADAVKFQMFQADRMYQKDP------------DVSIFSLVQSMEMPAEWILPLLDYCREKQVIFLSTVCDEGSADLLQ-STSPSAFKIASYEINHLPLLKYVARLNRPMIFSTAGAEISDVHEAWRTIR-----------------AEG---------NNQIAIMHCVAKYPAPPEYSNLSVIPMLAAAFPEAVIGFSDHSEHPTEAPCAAVRLGAKLIEKHFTIDKNLPGADHSFALNPDELKEMVDGIRKTEAELKQGITKPVSEKLLGSSYKTTTAIEGEIRNFAYRGIFTTAPIQKGEAFSEDNIAVLRPGQKPQGLHPRFFELLTSGVRAVRDIPADTGIV-

General information: TITO was launched using: RESULT: Template: 1VLI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1760 -96793 -55.00 -296.00 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : -55.00 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.347

(partial model without unconserved sides chains): PDB file : Tito_1VLI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1VLI-query.scw PDB file : Tito_Scwrl_1VLI.pdb: