Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNKLNKLMLGLGLTVASVAANAAGYGVIDLAKVVESSTYLKQQNASLNQSVKPTTTKLEQLGKELERLQRQAQTQGQKMKEDEIKKLQSQYQSKLNEFNSTQQGLQSRVQTSLQSMNTTFETRVKQAAEQLRKENNLDFILNKNSTVAYDAKYDLTDKMIQKVNSMK 1SG2 Chain:A ((38-122)) ---------------------------------------LENEFKGRASELQRMETDLQAKMKKLQSMKA---GSDRTKLEKDVMAQRQTFAQKAQAFEQDRA---RRSNEERGKLVTRIQTAVKSVANS-------------------------------------

General information: TITO was launched using: RESULT: Template: 1SG2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 94 1232 13.10 14.49 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.59 3D Compatibility (PKB) : 13.10 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.529

(partial model without unconserved sides chains): PDB file : Tito_1SG2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1SG2-query.scw PDB file : Tito_Scwrl_1SG2.pdb: