Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKLAIASALLSALAVSGAANAYQAEVGGSYNYLDPDNGSSVSKFGVDGTYYFNPVQTRNAPLAEAAFLNRASNVNAHVNYGDNSGTKDTQYGVGVEYFVPNSDFYLSGDVGRNEREIDNTNIDSKVTTYAAEVGYLPAPGLLLALGVKGYDEKD----------------GKD-GADPTVRAKYVTQVGQHDVNLEAYGAF------GDLDEYKVRGDYYIDKTLSLGVDYYNNDLT-DKDEFGINAKKFLNQQVSVEGRVGFGDNDNTYGVRAAYRF 2POR Chain:A ((147-301)) ----------------------------------------------------------------------------------------AQEMAVAAAYTFGN--YTVGLGYEKIDSPDTA--LMADMEQLELAAIAKF-GATNVKAYYADGELDRDFARAVFDLTPVAAAATAVDHKAYGLSVDST--FGA--TTVGGYVQVLDIDTIDDVTYYGLGASYDLGGGASIVGGIADNDLPNSDMVADLGVKFKF---------------------------

General information: TITO was launched using: RESULT: Template: 2POR.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 531 12707 23.93 97.00 target 2D structure prediction score : 0.43 Monomeric hydrophicity matching model chain A : 0.59 3D Compatibility (PKB) : 23.93 2D Compatibility (Sec. Struct. Predict.) : 0.43 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.112

(partial model without unconserved sides chains): PDB file : Tito_2POR.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2POR-query.scw PDB file : Tito_Scwrl_2POR.pdb: