Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPLIIGIDPGSR-----LTGYGIIEKDGSKLRFVDAGTIRTE-T-QEMPERLKRIFAGVERIVKFHGPT--EAAVEQVFMAQNPDSALKLGQARGAAIAALV--NLDLQVAEYTARQIKQSVVGYGAADKEQVQMMVMRLLNLTI-KPQ-ADAADALAAAICHAHASGSMSKLTVLNALGGMARGRSRSSSRRR 4KTZ Chain:B ((1-160)) MKKILAINFSTASKKGEGTGYAFRKDG--Q---VYVGSIKAYNPKKTAWERTFDIVNAIKDIIDEFDLKGYHLAIETPIMGRNRKHSITLANCNGYFIGAIDGLVNGYTFIDNSKWCSYHLISGKREQRKEESLELLKA-TGLVDSNCKDDNIADAYNILTYCEHL----------------------------

General information: TITO was launched using: RESULT: Template: 4KTZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 577 5019 8.70 34.14 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain B : 0.67 3D Compatibility (PKB) : 8.70 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.423

(partial model without unconserved sides chains): PDB file : Tito_4KTZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4KTZ-query.scw PDB file : Tito_Scwrl_4KTZ.pdb: