Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --MVSF--------LFIGLVVTILLTPGP---TNTLLASAGIQAGVKHSLKLIPAEVI--GYLIAIT---SWGVLLESVSHFI-PWLPAILKLM---SAGFILYLAFKLWM--------TSSEDIKLDRPLITPKALFL-------------ATLLNPKALLFASAIFPHAAWEI------PHLYFIHIVVFLSLITPIAFLWIMFGTVLISNKVTWLNQRNLQRI------AAFILTFFAMPIGYSAISSF---------------------------------------------- 1ZEL Chain:A ((2-297)) AMVVSPAGADRRIPTWASRVVSGLARDRPVVVTKEDLTQRLTEAGCGRDPDSAIRELRRIGWLVQLPVKGTWAFIPPGEAAISDPYLPLRSWLARDQNAGFMLAGASAAWHLGYLDRQPDGRIPIWLPPAKRLPDGLASYVSVVRIPWNAADTALLAPRPALLVRRRLDLVAWATGLPALGPEALLVQIATRPASFGPWADLVPHLDDLVAD-----CSDERLERLLSGRPTSAWQRASYLLDSGGEPARGQALLAKRHTEVMPVTRFTTAHSGESVWAPEYQLVDELVVPLLRVIGK

General information: TITO was launched using: RESULT: Template: 1ZEL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 881 -80051 -90.86 -416.93 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -90.86 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.291

(partial model without unconserved sides chains): PDB file : Tito_1ZEL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1ZEL-query.scw PDB file : Tito_Scwrl_1ZEL.pdb: