Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMDLFLNRKSFVVKSLAITVTALMMSGANAATSDKEEIRKLRQEVEALKALVQEQRQVQQQQQQVQQQQQVQLAEVKAQPQPAAAPASPLASLKSKTGADVNLYGFVRGDANYIIEGADNDFADVSKSNGKTQDKLRATAKTTRLGLDFTAPV-SGAKVGGKIELDFASSDNDKSENVRIRHAYLTYN----NWLFGQTTSNFLS-S-HAPEMIDFSTN-------------IGGGTTRIPQVRYNYKLAPATQLFVSAEEGNSSATGDSVKYRLPVLTAKVTQGYADGNGNASARAFVENYKSG---AGDDKTGWGIAAGTDFKVSDPLKLFADASYVVGNSNYLYGSNSAYTLVNDNIEQNEFVAVQVGGTYKILPNLRSTLAYGAQFADDGTDYAKNNVTANEKVQQAWINFIYTPVKPIDLGVEYVNGKRDTFEGKSYKDNRVGLMAKYSF 2POR Chain:A ((2-301)) ----------------------------------------------------------------------------------------------------VKLSGDARMGVMYNGDD------------------WNF-SSRSRVLFTMSGTTDSGLEFGASFKAHESVG------AETGEDGTVFLSGAFGKIEMGDALGASEALFGDLYEV-GYTDLDDRGGNDIPYLTGDERLTAEDNPVLLYTYSAGAFSVAASMSDGKVGE---TSEDDAQEMAVAAAYTF--GN--YTVGLGYEKIDSPDTALMADMEQLELA--AIAKFG-ATNVKAYYADGELDRDFARAVFD-LTPVAAAATAVDHKAYGLSVDSTFG-ATTVGGYVQVLDID--T---------IDDVTYYGLGASYDLGGGASIVGGIADNDLP------NSDMVADLGVKFKF

General information: TITO was launched using: RESULT: Template: 2POR.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1425 35932 25.22 129.72 target 2D structure prediction score : 0.48 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 25.22 2D Compatibility (Sec. Struct. Predict.) : 0.48 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.079

(partial model without unconserved sides chains): PDB file : Tito_2POR.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2POR-query.scw PDB file : Tito_Scwrl_2POR.pdb: