Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMRIGLFLLTNLAVLVVAGIILSLFGVGSYHGAGGLNLGNLLVICFVFGMVGSLVSLFMSKWMAKKTTGTELIDPNAPRNQAESWLLQTVAELSQRAGINMPEVGIFPS----YQSNAFATGWNKNDALVAVSSGLLERM-------------------NKDELRAVLAHEIGHVANGDMVTLALIQGVVNAFVMFFARVVGD---FIDRNVFGRQDNEAPGMGYFIITMVLDIVFGILASAIVMWFSRYREYRADEAGARLAGKQAMISALLRLQAETELPDQMPKEMKAFAIAEGKEQGFSLAALFQTHPTIEQRVAALHQLDCP 5SYT Chain:A ((234-474)) -------------------------------------------------------------------------------------LKEEIEVMAKSIDFPLTKVYVVEGSKRSSHS---------NK-RIVLFDTLLEEYSVLNKEEIKAKVKNKKQGCKNEEVLAVLGHELGHWKLGHTVKNIIISQMNSFLCFFLFAVLIGRKELFAAFGFYDS--QPTLIGLLIIFQFIFSPYNEVLSFCLTVLSRRFEFQADAFAKKLGKAKDLYSALIKLNKDNLG-FPVSDWL--------------FSMWHYSHPPLLERLQALKTMKQ-

General information: TITO was launched using: RESULT: Template: 5SYT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 594 -43650 -73.48 -232.18 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -73.48 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.403

(partial model without unconserved sides chains): PDB file : Tito_5SYT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5SYT-query.scw PDB file : Tito_Scwrl_5SYT.pdb: