Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKALVAVKRVVDANVKVRVKPDNSGVDLTNVKMSINPFCEIAVEEAVRLKEKGTVSEIVVVSIGPKEAQEQIRSAMALGADRGILVE--TT--DEIGALEVAKILKGVVDAEKPELILLGKQAIDDDSNQVGQMLGALLGAGQGTFASEVKVDGGKVQVTREIDGGLQTVELALPAIITTDLRLNEPRYAALPNIMKARKKPLDTKSPADYGVTAGTKLKTIKVEAPAERKAGVQVKSVDELVEKLKNEAKVI 1EFP Chain:D ((1-246)) MKVLVPVKRLIDYNVKARVKSDGSGVDLANVKMSMNPFDEIAVEEAIRLKEKGQAEEIIAVSIGVKQAAETLRTALAMGADRAILVVAADDVQQDIEPLAVAKILAAVARAEGTELIIAGKQAIDNDMNATGQMLAAILGWAQATFASKVEIEGAKAKVTREVDGGLQTIAVSLPAVVTADLRLNEPRYASLPNIMKAKKKPLDEKTAADYGVDVAPRLEVVSVREPEGRKAGIKVGSVDELVGKL-------

General information: TITO was launched using: RESULT: Template: 1EFP.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 1263 -26168 -20.72 -108.13 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain D : 0.91 3D Compatibility (PKB) : -20.72 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.91 QMean score : 0.472

(partial model without unconserved sides chains): PDB file : Tito_1EFP.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1EFP-query.scw PDB file : Tito_Scwrl_1EFP.pdb: