Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSSIFDLHIQVQPQHIDALGHVNNVMYVQWMQDVAAAHVETLGVGVTKYLELKHAMVAVEHHVQYRKAAFEGEEIVLRTWLDDI-NALYSFRQYAFFRPSDQAILFVGNTKWACIEIASG--RPKRMSPTFTQAYKPLDSSINPLDFTVSYA 2W3X Chain:A ((4-144)) -PRYYEYRHVVGFEETNLVGNVYYVNYLRWQGRCREMFLYEHAPEILDELRADLKLFTLKAECEFFAELAPFDRLAVRMRLVELTQTQMELGFDYLRLGGDDLLVARGRQRIACMRGPNGRTEPVRVPAGLVRAFAPFRSAT----------

General information: TITO was launched using: RESULT: Template: 2W3X.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 620 -67632 -109.08 -490.09 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -109.08 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.375

(partial model without unconserved sides chains): PDB file : Tito_2W3X.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2W3X-query.scw PDB file : Tito_Scwrl_2W3X.pdb: