Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNALTALSPLDGRYASKCDALRPFLSEFGLIHARVTVEVRWLQALSNRPEIVEVAPFSAETNAALDAI----VSNFSEEDANRIKEIERTTNHDVKAVEYFLKEKIAGIAELQNAGEFIHFACTSEDINNLSHALMLKNG-REVLVSSMKQILNAISALATTHAEQPMLSRTHGQTASPTTLGKEMANVAYRLARQIKQFENVELLGKINGAVGNYNAHLSAYPNVDWPAHSQAFVES-LGLTFNPYTTQIEPHDYMAELFDALRRFNTILIDFNRDVWGYISLGYFKQKLKEGEVGSSTMPHKVNPIDFENSEGNLGIANAVLAHLGEKLPISRWQRDLTDSTVLRNMGVGFAQSLIAFDACLKGIGKLELNANRLNEDLDQAQEVLAEPIQTVMRRYNVEKPYEKLKALTRGQAMTRDMMVDFVNGNELAQVPSEERARLAELTPATYTGNAAEQAKQINELISKI 4MX2 Chain:D ((32-478)) ---LYSLSPLDGRYKRDTTPLRAYFSEYALFKYRVQVEVLYFEALCK--EVPAITQLRGVTDAQLGELRATTFENFAVDDAKIIKGIEAVTNHDIKAVEYYLKDKMSACG-LEAEKEFIHFGLTSQDINNTSIPMLLRDALHHHYIPTLDQLIALLKS-KLPEWDVPMLARTHGQPASPTNLAKEFMVWIERLEEQRTMLLSIPNTGKFGGATGNFNAHLCAYPGVNWLDFGELFLSKYLGLRRQRYTTQIEHYDNLAAICDACARLHTILMDLAKDVWQYISLGYFDQKVRAGEVG-------VNPIDFENAEGNLGMSNAVLGFLSAKLPISRLQRDLTDSTVLRNLGVPLSHALIAFASLRRGIDKLLLNKDVIASDLEGNWAVVAEGIQTVLRREGYPKPYEALK-------VTEETVHRFV--QQL--ITEEVRQELLAITPFTYVGYTA-------------

General information: TITO was launched using: RESULT: Template: 4MX2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 1899 -11829 -6.23 -27.90 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain D : 0.82 3D Compatibility (PKB) : -6.23 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.82 QMean score : 0.559

(partial model without unconserved sides chains): PDB file : Tito_4MX2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4MX2-query.scw PDB file : Tito_Scwrl_4MX2.pdb: