Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAEFIAVILITILAVISPGADFAIVTKNSYLYGRKIGVFTSLGISLGVLVHVTYTLVAVAFVMTYTPQILNIVKYIGALYLIYIGYKTFTQKPVLETTALTAIGTFQAIKYGFFTNALNPKTTLFVISTYTQIVSLTTPKSILLAYGFFMSFAHFVWFSL-VAVLFSSMLLRQKMLAKQVQINRVIGSILCVLGVILLFTKFQ-- 5LNK Chain:J ((1-175)) MMTYIVFILSIIFVMGFVGFS----SKPSPIYG-GLGLIVSGGVGCGIVLNFGGSFLGLMVFLIYLGGMMVVFGYTTAMAT-----EQYPEVWVSNKVVLGTFITGLLMEFLMVYYVLKDKEVEIVFK--------------------FNGMGDWVIYDTGDSGFFSEEAMGIAALYSYGTWLVIVTGWSLLIGVVVIMEITRGN

General information: TITO was launched using: RESULT: Template: 5LNK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain J - contact count / total energy / energy per contact / energy per residue : 379 -56152 -148.16 -326.47 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain J : 0.69 3D Compatibility (PKB) : -148.16 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.134

(partial model without unconserved sides chains): PDB file : Tito_5LNK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5LNK-query.scw PDB file : Tito_Scwrl_5LNK.pdb: