Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGLNMPEPTSTTAATTTSLAAVSLLPFINGNALLGAVLGAAFVAYLEK-DLSAKQRIVFMLLCVGFGYLLGPEI-----------TSRTQYISSDATASMVAAIFAI-YILIKLLDWVKN--------STLAQLWKTFRGGGNS 5DJE Chain:A ((6-140)) FVADVPPPKKGTDYD----FYEAWGPVFEAEARFSK---KTPIPSLGNMDSSKKEVEQFYAFWHRFDSWRTFEFLDEDVPDDSSNRDHKRYIERKNKAARDKKKTADMARLVKLVERAVSEDPRIKMFKEEEKKEKERRKWE--

General information: TITO was launched using: RESULT: Template: 5DJE.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 275 4110 14.95 36.05 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : 14.95 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.265

(partial model without unconserved sides chains): PDB file : Tito_5DJE.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5DJE-query.scw PDB file : Tito_Scwrl_5DJE.pdb: