Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAKRKVVVALGGNAILSTD--ASAKAQQEALMETAKYLVKFIEQGDELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEKGIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGWRKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFASEKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPKVEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD 4OLC Chain:C ((4-317)) -AGKTVVIALGGNAMLQAKEKGDYDTQRKNVEIAASEIYKIHKAGYKVVLTSGNGPQVGAIKLQNQAA-AGVSPEMPLHVCGAMSQGFIGYMMSQAMDNVFCANNEPANCVTCVTQTLVDPKDQAFTNPTKPVGRFYTEQEAKDLMAANPGKILREDAGRGWRVVVPSPRPLEIVEYGVIKTLIDNNVLVICTNGGGIPCKRENKVISGVDAVIDKDLATSLLAKTLNSDYLMILTDVLNACI-------RKLEEIKLSEILALEKDGHFAAGSMGPKVRAAIEFTQAT-GKMSIITSLSTAVDALNGKCGTRIIKD

General information: TITO was launched using: RESULT: Template: 4OLC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1697 16026 9.44 52.54 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain C : 0.82 3D Compatibility (PKB) : 9.44 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.82 QMean score : 0.605

(partial model without unconserved sides chains): PDB file : Tito_4OLC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4OLC-query.scw PDB file : Tito_Scwrl_4OLC.pdb: