Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -------------------------------------------------------------------------------QVFGCFVQMSVRTQDSKDETNISSGGYLLTTNPGQLVFTDGNNNITVKVDKDCQSAGISYHRSEVHFTALQKLSENGGPFEFDQPLIELQ---------------------------------------------------------------------------------- 3MBR Chain:X ((3-238)) PVPTQGYRVVKRYPHDTTAFTEGLFYLRGHLYESTGETGRSSVRKVDLETGRILQRAEVPPPYFGAGIVAWRDRLIQLTWRNHEGFVYDLATLTPRARFRYPGEGWALTSDDSHLYMSDGTAVIRKL------------DPDTLQQVGSIKVTAGGRPLDN---LNELEWVNGELLANVWLTSRIARIDPASGKVVAWIDLQALVPDADALTDSTNDVLNGIAFDAEHDRLFVTGKRWPMLYEIRLTPLPH

General information: TITO was launched using: RESULT: Template: 3MBR.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain X - contact count / total energy / energy per contact / energy per residue : 248 23107 93.17 308.09 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain X : 0.55 3D Compatibility (PKB) : 93.17 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.55 QMean score : 0.380

(partial model without unconserved sides chains): PDB file : Tito_3MBR.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3MBR-query.scw PDB file : Tito_Scwrl_3MBR.pdb: