Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence WTGKSEASNRPPPGRRPEET---TWFTSDPSYACGVKIFRPGSEGSSGLAEITHLNFHEKIKVLKG-----QYECEVGPSCSTLRCTMPRGWRLDIFGKSFPTRRTTYFAENGNPEYNTLASEANIPLYDCRVVIRFPPTGGRDTGPLAEATHFNLGE----VVGVLGGRHTCQAQGQCANVNCNVPKGYTYYSLTGGPFQTKEKSHTFQSDA 1QR4 Chain:A ((1-175)) -----DNPKDLEVSDPTETTLSLRWRRPVAKFD-RYRLTYVSPSGK---KNEMEIPVDSTSFILRGLDAGTEYTISL-------------VAEKGR-HKSKPTTIKGSTVVGSPKGISFSDITEN------SATVSWTPPRSRVDS--YRVSYVPITGGTPNVVTVDGSKTRTKLVKLVPGVDYNV----NIISVKGF-EESEPISGILKT--

General information: TITO was launched using: RESULT: Template: 1QR4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 717 55728 77.72 341.89 target 2D structure prediction score : 0.48 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : 77.72 2D Compatibility (Sec. Struct. Predict.) : 0.48 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.215

(partial model without unconserved sides chains): PDB file : Tito_1QR4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1QR4-query.scw PDB file : Tito_Scwrl_1QR4.pdb: