Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence WFHEGLSRHQAENLLMG-KEVGFFIIRASQSSPGDFSISVRHEDDVQHFKVMRDNKGNYFLWTEKFPSLNKLVDYY 3N84 Chain:E ((9-84)) WFFGKIPRAKAEEMLSKQRHDGAFLIRESESAPGDFSLSVKFGNDVQHFKVLRDGAGKYFLWVVKFNSLNELVDYH

General information: TITO was launched using: RESULT: Template: 3N84.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain E - contact count / total energy / energy per contact / energy per residue : 261 -13947 -53.44 -185.96 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain E : 0.86 3D Compatibility (PKB) : -53.44 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.86 QMean score : 0.705

(partial model without unconserved sides chains): PDB file : Tito_3N84.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3N84-query.scw PDB file : Tito_Scwrl_3N84.pdb: