Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIQKHAIPILEFDDNPQAVIMPNHEGLDLQLPKKCVYAFLGEEIDRYAREVGANCVGEFVSATKTYPVYVINYKGEEVCLAQAPVGSAPAAQFMDWLI-GY--G-------------------------VEQIISTGTCGVLAD-IEENAFLVPVRALRDEGASYHYVAP-------------------------CRYMEMQPEAIAAIEEVLED---------RGIPYEEVMTWTTDGFYRETAE-------------KVAYRKE------EG---CAVVEMECSALAAVAQLRGVLWGELLFTADSLADLDQYDSRDWGSEAF-NKALELSLASVHHL 3BJE Chain:A ((36-348)) ------------------------KCKSDELADRIIFVGDPGRVDVISGYFDKDSIRA-SRDHREIRFATGTYKGTPVTVISTGMGVDNIEIVLNEIHALKEYDMERGQWRHRKGDADAPSAGPFFDPSTMKIIRLGTCGSPAESVPPLALAVTRHAIGMDNTSLYYSAGTRETSKDQQEIRRIVREQTGLRAIDIYTSMAHPNITKSICAACDAHNAATGSEADKQQYVIGTTATASGFYGCQGRRVGRFMKHLTVPNMVEELGSLKFNLSNGVEVVTNIEMETSAICYLSDMLGYQAGAACVVVSKRVGE-KKMFLGDQLDAAMKRCIKIILEALVS-

General information: TITO was launched using: RESULT: Template: 3BJE.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 843 -35490 -42.10 -156.34 target 2D structure prediction score : 0.46 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -42.10 2D Compatibility (Sec. Struct. Predict.) : 0.46 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.495

(partial model without unconserved sides chains): PDB file : Tito_3BJE.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3BJE-query.scw PDB file : Tito_Scwrl_3BJE.pdb: