Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSDCIFCKIIAGEIPASKVYEDEQVLAFLDISQVTLGHTLVVPKEHYRNLLEMDATSASQLFAQVPKVAQKVMKVTKAAGMNIISNCEEVAGQTVFHTHVHLVPRYSADDDLKIDFIAHEPDFDKLAQVAETIKNA 3P0T Chain:B ((7-108)) ---SIFTKIINRELPGRFVYEDDDVVAFLTIEPMTQGHTLVVPREEIDNWQDVDSAAFNRVMGVSQLIGKAVCKAFRTERSGLI-----IAGLEVPHLHVHVFPTRSLSD--------------------------

General information: TITO was launched using: RESULT: Template: 3P0T.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 437 -73315 -167.77 -718.77 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain B : 0.76 3D Compatibility (PKB) : -167.77 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.483

(partial model without unconserved sides chains): PDB file : Tito_3P0T.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3P0T-query.scw PDB file : Tito_Scwrl_3P0T.pdb: