Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNLNQLDIIVSNVPQVCADLEHILDKKADYADDGFAQFTIG-SHCLMLSQNHLVPLENFQSGIIIHIEV---EDVDQNYKRLNEL---GIKVLHGPTVTDWGTESLLVQGPAGLVLDFYRMK 3SK2 Chain:A ((12-131)) ITPNLQLVYVSNVERSTDFYRFIFKKEPVFVTPRYVAFPSSGDALFAIWSGGEEPVAEIPRFSEIGIMLPTGEDVDKLFNEWTKQKSHQIIVIKEPYTDVFG-RTFLISDPDGHIIRVCPL-

General information: TITO was launched using: RESULT: Template: 3SK2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 384 -27659 -72.03 -244.77 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : -72.03 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.629

(partial model without unconserved sides chains): PDB file : Tito_3SK2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3SK2-query.scw PDB file : Tito_Scwrl_3SK2.pdb: