Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLKSEKQSRYQMLNEELSFLLEGETNVLANLSNASALIKSRFPNTVFAGFYLFDGK--ELVLGPFQGGVSCIRIALGKGVCGEAAHFQETVIVGDVTTYLNYISCDSLAKSEIVVPMMKNGQLLGVLDLDSSEIEDYDAMDRDYLEQFVAILLEKTTWDFTMFEEKS 3MMH Chain:B ((12-162)) ------AALYREVLPQIESVVADETDWVANLANTAAVLKEAF-GWFWVGFYLVDTRSDELVLAPFQGPLACTRIPFGRGVCGQAWAKGGTVVVGDVDAHPDHIACSSLSRSEIVVPLFSDGRCIGVLDADSEHLAQFDETDALYLGELAKILEKRFEA---------

General information: TITO was launched using: RESULT: Template: 3MMH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 746 -43129 -57.81 -289.46 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain B : 0.81 3D Compatibility (PKB) : -57.81 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.571

(partial model without unconserved sides chains): PDB file : Tito_3MMH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3MMH-query.scw PDB file : Tito_Scwrl_3MMH.pdb: