Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNKSEHRHQLIRALITKNKIHTQAELQALLAKNDIQVTQATLSRDIKNMNLSKVREEDSA---YYVLNNGS----ISKWEKRLELYMEDALVWMRPVQHQVLLKTLPGLAQSFGSIIDTLSFPDAIATLCGNDVCLIICEDADTAQKC---FEELKKFAPPFFFEE 3FHZ Chain:F ((20-170)) -----GRQARIVAILSSAQVRSQNELAALLAAEGIEVTQATLSRDLEELGAVKLRGADGGTGIYVVPEDGSPVRGVSGGTDRMARLLGELLVSTDDSGNLAVLRTPPGAAHYLASAIDRAALPQVVGTIAGDDTILVVAREPTTGAQLAGMFENLR----------

General information: TITO was launched using: RESULT: Template: 3FHZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain F - contact count / total energy / energy per contact / energy per residue : 470 -30302 -64.47 -214.91 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain F : 0.78 3D Compatibility (PKB) : -64.47 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.513

(partial model without unconserved sides chains): PDB file : Tito_3FHZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3FHZ-query.scw PDB file : Tito_Scwrl_3FHZ.pdb: