Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTKKIVAIWAQDEEGLIGKENRLPWHLPAELQHFKETTLNHAILMGRVTFDGMGRRLLPKRETLILTRNPEEKIDGVATFQDVQSVLDWYQDQEKNLYIIGGKQIFQAFEPYLDEVIVTHIHARVEGDTYFPEELDLSLFETVSSKFYAKDEKNPYDFTIQYRKRKEV 3SA2 Chain:B ((5-156)) ---RVSFMVAMDENRVIGKDNNLPWRLPSELQYVKKTTMGHPLIMGRKNYEAIGRP-LPGRRNIIVTRNEGYHVEGCEVAHSVEEVFELCKNEEE-IFIFGGAQIYDLFLPYVDKLYITKIHHAFEGDTFFP-EMDMTNWKEVFVEKGLTDEKNPYTY----------

General information: TITO was launched using: RESULT: Template: 3SA2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 666 -111663 -167.66 -734.62 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain B : 0.84 3D Compatibility (PKB) : -167.66 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.84 QMean score : 0.583

(partial model without unconserved sides chains): PDB file : Tito_3SA2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3SA2-query.scw PDB file : Tito_Scwrl_3SA2.pdb: