Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---------MIFATSQVNPLTNSSVRN-------LRPKQRRLFM-------FWNLVHYEFKNVNKWYLALYAAVLVLSALIGIQTQGFKNLPYQESQATMLLFLATVFGGLMLTLAISTIFLIIKRFKGSVYDRQGYLTLTLPVSEHHIITAKLIGAFIWSLISTAVLALSAVIILALTAPEWIPLSYVITFVETH----LPQIFLTGISFLLNTISGILCIYLAISIGQLFNEYRTALAVAVYIGIQIVIGFIELFFNLSSNFYVNSLVGLNDHFYMGAGIAIVEELIFIAIFY---LGTYYILRNKVNLL 5HJQ Chain:A ((78-367)) ALRSYTEIQQLLQQGKKRDVKNILRENSWPINSPIRAQLWPMLCGQHLDGFYWEMVHQVFGTTELSEKPIMLPAFVDATHC---LPYHLTSTGRAVADRIVNVLGYDCPDITYSPVLYPITSILLHF--------------MSEEEAYICLAGLVGSKEKVFINQTKLQHEVTWKTVMQIAKKHTKSATSYFQRICPGLKLERIFM---DWCWWILAGLPFQHLVRIMDCYFHEGIKVLYRVALVILNLFHKECQSNNEWSPDNIKNDIGNALIKFCKK--IPVSPAKLLHAAFSIRGLSTQYISR------

General information: TITO was launched using: RESULT: Template: 5HJQ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1083 -120624 -111.38 -474.90 target 2D structure prediction score : 0.46 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : -111.38 2D Compatibility (Sec. Struct. Predict.) : 0.46 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.293

(partial model without unconserved sides chains): PDB file : Tito_5HJQ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5HJQ-query.scw PDB file : Tito_Scwrl_5HJQ.pdb: