Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDNRPIGFLDSGVGGLTVVRELMRQLPHEEIVYIGDSARAPYGPRPAEQIREYTWQLVNFLLTKDVKMIVIACNTATAVVWEEIKAQLDIPVLGVILPGASAAIKSSQGGKIGVIGTPMTVQSDIYRQKIHDLDPDLQVESLACPKFAPLVESGALSTSVTKKVVYETLRPLVGK-VDSLILGCTHYPLLRPIIQNVMGPKVQLIDSGAECVRDISVLLNYFEINRGRDAGPLH--HRFYTTASSQSFAQIGEEWLEKEIHVEHVEL 2DWU Chain:C ((10-267)) -----IGVLDSGVGGLTVASEIIRQLPKESICYIGDNERCPYGPRSVEEVQSFVFEMVEFLKQFPLKALVVACNTAAAATLAALQEALSIPVIGVIHPGARAAIKVTKKGKIGVIGTVGTIQSNMYEKALHELDTYLKVHSHACPTLATVVENRLEDTAYVTQQVKQALLPLTKEDIDTLILGCTHYPLLESYIKKELGEDVTIISSAEETAIELSTILQH----KGILADNLNPKHRFFTTGSVSSFEHIAERWLGYQISVDCVDL

General information: TITO was launched using: RESULT: Template: 2DWU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1513 -168817 -111.58 -662.03 target 2D structure prediction score : 0.74 Monomeric hydrophicity matching model chain C : 0.84 3D Compatibility (PKB) : -111.58 2D Compatibility (Sec. Struct. Predict.) : 0.74 1D Compatibility (Hydrophobicity) : 0.84 QMean score : 0.580

(partial model without unconserved sides chains): PDB file : Tito_2DWU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2DWU-query.scw PDB file : Tito_Scwrl_2DWU.pdb: