Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSHV--RVQIMNQFHRKSHEYKAIKRYWKLIQQDSRKLSDKRFYRPTFRMHLTNKEILNKLLSYSEDLKHHYQLYQLLLFHFQNKEPEKFFGFIEDNLKQVHPLFQTVFKTFLKNKEKIVNALQLPYSNAKLEATNNLIKLIKRNAFGFRNFENFKKRIFIALNIKKERTKFVLSRA 1W4S Chain:A ((26-171)) MYHVGDYVYVEPAEANLQPHIVCIERLWED-SAGEKWLYGCWFYRPNETFHLATRKFLEKEV-FKSDYYNKVPVSKIL---------GKCVVMFVKEYFKLCPE---------NFRDEDVYVCESRYS-----AKTKSFKKIKLWTMPVSSVRFVPRDV--PLPVVRVASVFA----

General information: TITO was launched using: RESULT: Template: 1W4S.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 630 -9527 -15.12 -66.16 target 2D structure prediction score : 0.13 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -15.12 2D Compatibility (Sec. Struct. Predict.) : 0.13 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.068

(partial model without unconserved sides chains): PDB file : Tito_1W4S.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1W4S-query.scw PDB file : Tito_Scwrl_1W4S.pdb: