Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MANRKIVVALGGNAILSSDPSAK--AQQEALVETAKHLVKLIKNGDDLIITHGNGPQVGNLLLQHLASDSEKNPAFPLDSLVAMTEGSIGFWLKNALQNALLDEGIEKNVASVVTQVVVDKNDPAFVNLSKPIGPFYSEEEAK-AEAEKSGATFKEDAGRGWRKVVASPKPVDIKEIETIRTLLNNGQVVVAAGGGGIPVVKENNGHLTGVEAVIDKDFASQRLAELVDADLFIVLTGVDYVFVNYNKPNQEKLEHVNVAQLEEYIKQDQFAPGSMLPKVEAAIAFVNGRPEGK-AVITSLENLGALIESESGTIIEKG 4OLC Chain:C ((4-316)) -AGKTVVIALGGNAMLQAKEKGDYDTQRKNVEIAASEIYKIHKAGYKVVLTSGNGPQVGAIKLQNQAA-AGVSPEMPLHVCGAMSQGFIGYMMSQAMDNVFCANNEPANCVTCVTQTLVDPKDQAFTNPTKPVGRFYTEQEAKDLMAANPGKILREDAGRGWRVVVPSPRPLEIVEYGVIKTLIDNNVLVICTNGGGIPCKRENK-VISGVDAVIDKDLATSLLAKTLNSDYLMILTDVLNACI-------RKLEEIKLSEILALEKDGHFAAGSMGPKVRAAIEFT--QATGKMSIITSLSTAVDALNGKCGTRIIK-

General information: TITO was launched using: RESULT: Template: 4OLC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1652 18741 11.34 62.05 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain C : 0.82 3D Compatibility (PKB) : 11.34 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.82 QMean score : 0.591

(partial model without unconserved sides chains): PDB file : Tito_4OLC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4OLC-query.scw PDB file : Tito_Scwrl_4OLC.pdb: