Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLIQLYLLKLELFWFELHQHYHFSEPQKILNELIAAYSAAYSEKQRAYHTVQHLYECLSLIETVQSE--------LNDPYAVALALWFHDVVYEPQAL------------------DNELKSAELFEQLMAQ-DL------QL----DTMQKIKRWILATQKHAP------------------TDETDLQFLLDIDLAILAATPERFMQYEQQIQQEYSWADPEVYSIKRKEVLMNFYQSEPLYQTAYFQKNFELNAKQNLKKILE 5B25 Chain:A ((57-249)) -----------------------VFLMSFLDALETG----YGKYKNPYHNQIHAADVTQTVHCFLLRTGMVHCLSEIELLAIIFAAAIHDYEHTGTTNSFHIQTKSECAIVYNDRSVLENHHISSVFRLMQDDEMNIFINLTKDEFVELRALVIEMVLATDMSCHFQQVKTMKTALQQLERIDKPKALSLLLHAADISHPTKQWLVHSRWTKALMEEFFR----------------------------------------------

General information: TITO was launched using: RESULT: Template: 5B25.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 500 247 0.49 1.79 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.57 3D Compatibility (PKB) : 0.49 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.57 QMean score : 0.482

(partial model without unconserved sides chains): PDB file : Tito_5B25.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5B25-query.scw PDB file : Tito_Scwrl_5B25.pdb: