Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQIGIPTETVVGENRVAATPETVKKLISAGHSVVIERGAGVKAAYIDSAYEQVGATITD---DAYTGSQIILKVRAPQGGE-----IQKLAANTTVVAMFDPYRNTEL-DQFANQQVSAFALELLPRTLSRAQNMDVLSSQANLAGYKSVLLAAAEYQRMFPMLMTAAGTVKPARVVIMGVGVAGLQAVATAKRLGAIVEATDLRPTAKDQVESLGGKWLDVPMSEEEQQRAADAAKNGYGWMPGEQYIKDQAAIVDKAVSNADIVITTALLPGRDAPRLIKAETVAKMKPGSVILDMAVETGGNVEGSKVGETVVTENGVKILGIPNIPATVATEASALYARNVFNFVETLFDKEKNFAINQEDEIQKALLVTHGGQVLLKRG 1U28 Chain:B ((1-375)) MKIAIPKERRPGEDRVAISPEVVKKLVGLGFEVIVEQGAGVGASITDDALTAAGATIASTAAQALSQADVVWKVQRPMTAEEGTDEVALIKEGAVLMCHLGALTNRPVVEALTKRKITAYAMELMPR-ISRAQSMDILSSQSNLAGYRAVIDGAYEFARAFPMMMTAAGTVPPARVLVFGVGVAGLQAIATAKRLGAVVMATDVRAATKEQVESLGGKFITV---D----------------------RKKQAEAVLKELVKTDIAITTALIPGKPAPVLITEEMVTKMKPGSVIIDLAVEAGGNCPLSEPGKIVV-KHGVKIVGHTNVPSRVAADASPLFAKNLLNFLTPHVDKDKTLVMKLEDETVSGTCVTRDGAIV----

General information: TITO was launched using: RESULT: Template: 1U28.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2008 -5340 -2.66 -15.52 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain B : 0.85 3D Compatibility (PKB) : -2.66 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.85 QMean score : 0.564

(partial model without unconserved sides chains): PDB file : Tito_1U28.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1U28-query.scw PDB file : Tito_Scwrl_1U28.pdb: