Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MHLAQRLAAVVLTLGLSAGLVGCGFHLKGTNPTATPLVYKKLSL-ELPAKTDDLETQLKVYLTANGVQLSND----NDAYVLRVLEYTPRRQLL----NGKLTEVLLRLTVTFQIEDRQGNKITEPRTLTAARSYQYDLATVNTENQQESYLQRIVIDDLAQQITRQISANRLPKAQP 4NHR Chain:A ((15-151)) --------------------------------------MKVMILDSGDPN-GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIMTVNATVLIP-GRDIY-PISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLPSIRAADIRS

General information: TITO was launched using: RESULT: Template: 4NHR.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 520 14981 28.81 117.04 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : 28.81 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.450

(partial model without unconserved sides chains): PDB file : Tito_4NHR.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4NHR-query.scw PDB file : Tito_Scwrl_4NHR.pdb: