Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSSAVVVSSENLDGQQQSSSTPASPAAEKVNLLGMSRAELEKFFEDIGEKKFRAGQVMKWIHQYFVTDFAEMTNISGKLRAKLEQICEIKAPEVVHRHYSKDGTRKWVFRVGEGSGSLVETVLIPAEDKTGSRKTLCISSQVGCALDCSFCSTGKQG--------FQRDLTPDEIIGQLWMANYSYMEEVPVAERERSVTNVVMMGMGEPLLNYDAVLSSMHIMLDDFAYGMSKRRVTLSTSGVVP-K-IDQLAKDIDV-ALAISLHAPNDELRNELVPINKKYPLAQLIAACQRYIAKDGNESARKHVTIEYVMLEGVNDQPEHAQQLLKLLKNLPSKINLIPFNPFPHAPYGRSSRNRIISFQKTLSDAGFVCTIRQTRGDDIDAACGQLVGQVADRTRRAEQWQKKVAQRQEILRTQG 2FB2 Chain:A ((14-202)) -------------------------------------------------------------------------------------------------------------------------------------RDLALSVTDRCNFRCDYCMPKEVFGDDFVFLPKNELLTFDEMARIAKVYAE------------LGVKKIRITG-GEPLMRRD-LDVLIAKLNQIDGI----EDIGLTTNGLLLKKHGQKL-YDAGLRRINVSLDAIDDTLFQSINNR--NIKATTILEQIDYATSI------GLNVKVNVVIQKGIN--DDQIIPMLEYFKDKHIEIRFIEFMDVGND----------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2FB2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 824 -4162 -5.05 -23.38 target 2D structure prediction score : 0.78 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -5.05 2D Compatibility (Sec. Struct. Predict.) : 0.78 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.544

(partial model without unconserved sides chains): PDB file : Tito_2FB2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2FB2-query.scw PDB file : Tito_Scwrl_2FB2.pdb: