Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKLQTIACAVAIATGGLFFSHTMNEARAATNTAAVSQSIQPTQEQALVARQLATLVDRQHYLNMRLDANTSNRILDMYLDSLDPDHSLFLDAEVQNYKKLYGSNFGASLKAGNLTGPFAIHQQYRERLKQFYEFMLAELKKPQNLKQPNTFIEVDREKAPYFKTSAEQQNHWRKMLVSQLINLTISREEEQAKQKALKENPSLADGQDLTGPEDLTPAQTLTKRYTRQLERISRVKSDDVLDKTLNAMLATYDPHSNYYPPIDAIELNRQTTLQLEGIGVSIRPERGNE--D-YTKIETIVEGGPASKSGQVKSGDRIVGVAQEG-GKMIDVVGWSSSEIVGLIRGKRGTKVTLKLLGAGAS-MSQARNVTLVRDVIQEEDA-G-----VRSRTVEVTRDGKKHLLGVIEIPSFYFDYRSRRAGQQYRSVSEDTANAFEALKAKKVEGIIIDLRNDPGGSLEEVARMLGQVIKSGPVVQIRDGNGNVSVFEDNDGGQQIYTGPLAVLVNLASASASEIYSAAIQDYERGIIIGSTTTGKGTAQVQLDTLAYGQATLTQRKFYRVTGGSTQNKGVVPDIKLVDIYNEEFGERKSKNALKWDTIPTAPFKREGSVQPYVAKLSQLSEQRVAVDPQFKYLNKRTAIAKVTSDQKQVVLDIDKRRAELLSLEKQTLDAENERRIATGQKPFPNWESYQASLDALAESRAKMKANQRPALPEEETFVNEAANVLMDYAKLQNR 1FCF Chain:A ((30-376)) -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------WFKLRETYLKKE---PMDRRAQTYDAIRKLLAVLDDPFTRFLEPSRLAALRRGTAGSVTGVGLEITY---DGGSGKDVVVLTPAPGGPAEKAG-ARAGDVIVTVDGTAVKGLS------LYDVSDLLQGEADSQVEVVLHAPGAPSNTR--TLQLTRQKVTINPVTFTTCSNVAAAALPPGA--AKQQLGYVRLATFNSNT------------TAAAQQAFTELSKQGVAGLVLDIRNNGGGLFPAGVNVARMLVDRGDLVLIADSQGIRDIYSADG-NSIDSATPLVVLVNRGTASASEVLAGALKDSKRGLIAGERTFGKGLIQTVVDLSDGSGVAVTVARYQTPAGVDINKIGVSPDVQLDPEVLP----TDLEGVCR--------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1FCF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1825 30174 16.53 89.80 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 16.53 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.399

(partial model without unconserved sides chains): PDB file : Tito_1FCF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1FCF-query.scw PDB file : Tito_Scwrl_1FCF.pdb: