Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLLVYTLIAIVALIVMIAKFRIYPFLVLIIVSLGLALAVGMPMDGIVKSYEAGTGKTLGHLAIVIALGSMLGKMMAESGGAERIAIALIKWFGE-KH-IHWAMMFIALIVGLPVFFEVGFVLLIPIAFNIAKRTGKSLLI-----VGLPMVAGLSVVHGLIPPH-PAALLAVQAYHADIGKTIMYSLLVGVPTAVVAGPLYALWINKYVKLPENNPLAKQFVEADANNTRELPSFGITLFTIMLPVALMLVGSWADVFFAPKTFPNELLRFIG-TSDIALLIAVLVSFITFGTMQGFNREQIEKFCGGCLASIAGILLIVGAGGGFGGILRDSGISNEIVSTALKA-N-LSPLLLG--WLVAAIIRLATGSATVAMATACSIVAPIAAAAGVVVRPELLVLATGSG--SLIFSHVNDAGFWLIKEYFGMTVGQTLKTWSVLETIISVLGLSFTLLLSAVL 5ULE Chain:C ((35-444)) ---------LAFIAVLWLTEALHVTVTAILVPVMAVFFGIFET----QAALNNFAN---SIIFLFLGGFALAAAMHHQGLDKVIADKVLAMAQGKMSVAVFMLFGVTALLSMWISNTATAAMMLPLVLGVLSKVDADKQRSTYVFVLLGVAYSASIGGIATLVGTGPNAIAAAEVGLSFTDWMKFGLPTAMMMLPM-AIAILYFLLKPTLNGMF-EL-------DRAPVNWDKGKVVTLGIFGLTVFLWIFSSPINA----------ALGGFKSFDTLVALGAILMLS-FA--R-VVHWKEIQKTAD------WGILLLFGGGLCLSNVLKQTGTSVFLANALSDMVSHMGIFVVILVVATFVVFLTEFTSNVATTTLLIPVFATVAEAFG--MSPVLLSVLIAVAASCAFMLPVATPPNAIVFASGHIKQSEMMRVGLYLNIACIGLLTAIAMLFW---

General information: TITO was launched using: RESULT: Template: 5ULE.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 2042 -270434 -132.44 -684.64 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain C : 0.65 3D Compatibility (PKB) : -132.44 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.399

(partial model without unconserved sides chains): PDB file : Tito_5ULE.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5ULE-query.scw PDB file : Tito_Scwrl_5ULE.pdb: