Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MELRHLRYFVAVVEEQSFTKAAEKLFIAQPPLSRQIQNLESELGIQLFERGSRPLQTTPAGHFFYQHAVKLLSNAEEIKSMTKRIGLIERSMTIGFVGSLLYGLLPRIIYLFRQQQPHLNIQLMELSTTEQLQALKEGRIDVGFGRLRISDPAVRRILLRKERLVVAAHTSHPIAQ-RTEGVYLADLIDEKMFMYPTSLKPNFSTQLLNIFAEHSLVPKNMHEIREIQLALGLVAAGEGICIIPASADTIRFPHLNYIPILDNGAVSPIFITARAMDRSEDLQSLFDCIYQVYDLEGIPYQRTVFTLDQNPIDDSNGIDF 3GLB Chain:B ((2-205)) -----------------------------------------------------------------------------------------QTLRIGYVSSLLYGLLPEIIYLFRQQNPEIHIELIECGTKDQINALKQGKIDLGFGRLKITDPAIRHIVLHKEQLKLAIHKHHHLNQFAATGVHLSQIIDEPMLLYPVSQKPNFATFIQSLFTELGLVPSKLTEIREIQLALGLVAAGEGVCIVPASAMDIGVKNLLYIPILDDDAYSPISLAVRNMDHSNYIPKILACVQEVF---------------------------

General information: TITO was launched using: RESULT: Template: 3GLB.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1061 -121556 -114.57 -598.80 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain B : 0.75 3D Compatibility (PKB) : -114.57 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.557

(partial model without unconserved sides chains): PDB file : Tito_3GLB.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3GLB-query.scw PDB file : Tito_Scwrl_3GLB.pdb: