Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKYCQFFSVLALGLSAASCAVTSGLQTYDIPSEGVYKTDLGTTVNVVKISQETLPAIQPAQIDYQRDYASLFKNQQTIYRLSPGDVLSIQLWAYPEIAPPVNNISNEQSVQAYGYPIDQTGYIQFPLVGRYKAAGKTLAQVNRELHSQLARFLKNPDVVVRVVSYEGQRFSVQGSVTKGGQF---YLSDQPVSIYTALGMAGGVTTTGDNTYIQLIRNGRT--------------------------------------------YNLNTIDLEKAGYSLHKLLVQPNDTIYVSTRENQKIYVMGESGKNQALPMRDQGMSLTDALGESLGINPLSGSASRIYVVRTNPNDRTTEIYHLNLMSIGDFGLANQFRLRSNDVVYVDATGL------TRWQRIVNQIIPFSNALYNIDRLGQ 3P42 Chain:A ((4-228)) ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------GMVTIYLPGEQQTLSVG-PVENVAQLVTQPQ-LRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKSVRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRP-VTITLLGAVSGAGQLPWLAG-RSVTDYLQDHPRL-A-GADKNNVMVITPEG---ETVVAPVALW------NKRHVEPPPGSQLWLGFSAHVLPEKYADLNDQIVSVLTQRVPE--------

General information: TITO was launched using: RESULT: Template: 3P42.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 596 -266 -0.45 -1.55 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain A : 0.56 3D Compatibility (PKB) : -0.45 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.56 QMean score : 0.283

(partial model without unconserved sides chains): PDB file : Tito_3P42.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3P42-query.scw PDB file : Tito_Scwrl_3P42.pdb: