Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNENMKRLVDIVISLIALTVLSPIFLIVAYKVRKNLGSP-IFFYQERPGKDGKLFKMIKFRSMKDA-FDAQGNPLPDEARITPFGQKLRSTSLDEMPQLI-NVLKGDMSVVGPRPMLKDFVALYSPEQARRLEARPGMTGLAQVSGRNELDYEERFKCDVWYVDNHNVWVDFKIMFKTVKVMLKREGINAPGHV-GPSLFKGNDTQENIDSSVK 1KCW Chain:? ((347-560)) IRGKHVRHYYIAAEEIIWNYAPSGIDIFTKENLTAPGSDSAVFFEQGTTRIGGSYKKLVYREYTDASFTNRKERGPEEEHLGILGPVIWAEVGDTIRVTFHNKGAYPLSI--------EPIGVRFNKNNEGTYYSPVPPSASHVAPTETFTYEWTVPKEVGPTNADPVCLAKMYYSAVDPTKDIFTGLIGPMKICKKGSLHANGRQK-------

General information: TITO was launched using: RESULT: Template: 1KCW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 932 26164 28.07 134.17 target 2D structure prediction score : 0.30 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : 28.07 2D Compatibility (Sec. Struct. Predict.) : 0.30 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.118

(partial model without unconserved sides chains): PDB file : Tito_1KCW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1KCW-query.scw PDB file : Tito_Scwrl_1KCW.pdb: