Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSKTSVIYPGTFDPITNGHVDLVTRASRMFDEVVVAIAIGHHKNPLFSLEERVALAQSSLGHLSNVEFVGFDGLLVNFFKEQKATAVLRGLRAVSDFEYEFQLANMNRQLDPHFEAVFLTPSEQYSFISSTLIREIARLKGDVTKFVPQAVVEAFERKHQQGW 5TS2 Chain:F ((12-166)) -----VLYPGTFDPITKGHGDLIERASRLFDHVIIAVAASPKKNPLFSLEQRVALAQEVTKHLPNVEVVGFSTLLAHFVKEQKANVFLRGLRAVSDFEYEFQLANMNRQLAPDVESMFLTPSEKYSFISSTLVREIAALGGDISKFVHPAVADALAERFK---

General information: TITO was launched using: RESULT: Template: 5TS2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain F - contact count / total energy / energy per contact / energy per residue : 726 -116878 -160.99 -754.05 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain F : 0.91 3D Compatibility (PKB) : -160.99 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.91 QMean score : 0.623

(partial model without unconserved sides chains): PDB file : Tito_5TS2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5TS2-query.scw PDB file : Tito_Scwrl_5TS2.pdb: