Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTRKNAIAALLLLPSFSYAATVLSAPPELNNKSYVLMDYETGQILASKNENEKLAPASMTKMMTSYIIEQKLLKGELTENEQVRMNESAWCKGSSS---ESCMYVPLNGTATVLEMLRGIIIQSGNDASKAMAEHIAGNEGTFAHMMNQEAKRIGMTNTQFINSTGMPAEGHYSTAKDMAVLAQHIIKDSSKYYPIYSEKEFTFNGIKQGNRNALLY-TDPSVDGLKTGHTDEAGYCLTTSSKRGPMRLISVIFGTPSMSERADQTRALLAWGFANFETANVQPANQVLAKAKVWFGKENEVQVGLAENFNVTMPKGKADGIK-TQLVVQPNLNAPLQKGQVVGKLVASLDGKVIAEKPLVALKPVEEAGFFARLIDHIKQFFSNLF 3IT9 Chain:B ((9-352)) --------------------------PSVDARAWILMDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGNPALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAKKLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQPNRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEKLLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASYTLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGG-----------------

General information: TITO was launched using: RESULT: Template: 3IT9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2008 59637 29.70 175.92 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain B : 0.78 3D Compatibility (PKB) : 29.70 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.493

(partial model without unconserved sides chains): PDB file : Tito_3IT9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3IT9-query.scw PDB file : Tito_Scwrl_3IT9.pdb: