Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKNKTIILGGFTEMKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAALITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDG-FQYFQS-SNESDYIPNIDQALNAGFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVAAAYTTETNVVGFIGGVKG-----------EVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDKGRSIAQGMYA--QNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYTLNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDEAKKAVDEAKEKIISGDVKVPETPEEN 5UFH Chain:A ((66-321)) -----------------------------------------------------GLLGLAI-FD--FSQPFASYLADQIIVCAREH------HYGVIINTYGQNEHGLARAMRQANNLAADGWIVFADHAMGQ--KM--LDQSYPLVLTGDWDA-YGKVDQVTMPNVEAMRYTTNRLLDS-GYRSIALFGADGSLGARHYRQATEGTQELRVQGYMQAYEEH-G---IEARMDMLFSGGLLTSDSGVRATNLMMDQGVRPDAIICLNDAMALGALHQLQVRGIRVPDDVQVVGFDNVPE--------ATYANPSLTTIDPHIDSYARHAVDMLIE------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5UFH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1274 8994 7.06 37.63 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : 7.06 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.409

(partial model without unconserved sides chains): PDB file : Tito_5UFH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5UFH-query.scw PDB file : Tito_Scwrl_5UFH.pdb: